组织芯片的基本应用范围

Basic applications of tissue microarray

目的 探索组织芯片的基本应用范围。方法 将HE染色、组织化学、免疫组织化学、原位杂交和荧光原位杂交技术应用于组织芯片 ,了解这些技术应用于组织芯片的可行性和有效性。结果 这些检测技术在组织芯片中均有清晰、定位明确的着色。结论 HE染色、组织化学、免疫组化、原位杂交和荧光原位杂交技术可有效地用于组织芯片 ,因此 ,组织芯片可用于组织形态观察、组化特性分析以及蛋白和核酸 (RNA和DNA)在组织细胞中的定位性研究。

Objective To clarify basic application scopes of tissue microarray. Methods Hematoxylin and eosin stain (HE), histochemistry, immunohistochemistry, in situ hybridization and fluorescence in situ hybridization (FISH) techniques were used on tissue arrays to show histological morphology and distribution of protein antigens, RNA and DNA in cells. Results Cytoplasm and nuclei of cells were clearly stained as red and blue, respectively, by HE.Representative Masson stain gave rise to clear green, red and dark red in goblet cells, smooth muscle cells and glands, respectively, in small intestine.Cytokeratin (AE1/AE3) was expressed in almost all epithelia.Epstein-Barr virus encoded RNAs (EBERs) were detected in nuclei of Hodgkin lymphoma cells using in situ hybridization.Centromeres of chromosome 7 were stained as light green dots in nuclei of lymphocytes using FISH. Conclusion Techniques including HE, histochemistry, immunohistochemistry, in situ hybridization and FISH can be effectively applied on tissue microarrays.Therefore, tissue array can be used for morphology, histochemical analysis, protein, and nucleotides (RNA and DNA) in situ detection.