不育症患者精浆IL-2,IL-6,IL-8,TNF-α测定的临床意义

Clinical Significance of Determination of Semen Plasma IL-2, IL-6, IL-8 and TNF-α Contents in Infertile Males

目的 :观察精浆中白细胞介素 - 2 (IL - 2 )、白细胞介素 - 6 (IL - 6 )、白细胞介素 - 8(IL - 8)、肿瘤坏死因子 -α(TNF -α)等细胞因子对男性生殖的影响。方法 :应用放射免疫分析 (RIA)对 1 2 6例不育男性和 2 0例正常生育男性精浆IL - 2、IL - 6、IL - 8、TNF -α水平进行了检测。结果 :不育症组精浆IL - 2、IL - 6、IL -8、TNF -α含量均高于生育组 (p <0 0 5或 p <0 0 1 ) ;精浆中IL - 2、IL - 6、IL - 8、TNF -α含量在不育症组的WBC精液组与非WBC精液组之间均存在显著性差异 (p <0 0 5或 p <0 0 1 )。另外 ,精浆中TNF -α含量在精子活动力、活动率正常与减少之间 ,IL - 8含量在精子活动率正常与减少之间均存在显著性差异 (p <0 0 5或p <0 0 1 )。结论 :检测精浆中IL - 2、IL - 6、IL - 8、TNF -α等细胞因子的含量可以反映男性不育症患者的状态 。

Objective To explore the influence of high semen plasma contents of the cytokines (IL-2, IL-6, IL-8, TNF-α) on male fertility. Methods Semen plasma levels of IL-2, IL-6, IL-8, and TNF-α were determined with RIA in 126 infertile and 20 fertile males. Results Semen plasma contents of the 4 cytokines in infertile subjects were significantly higher than those in fertile ones ( p<0 05 or p<0 01 ). Infertile subjcets with leucocytospermia (WBC>4/HP, n=15 ) had significantly higher contents of cytokines than those without leucocytospermia (WBC<4/HP, n=111 ). Besides, TNF-α contents in subjects with lower sperm activity and less motility rate as well as IL-8 contents in subjects with less sperm motility rate were both significantly higher than those in subjects with more normal sperms ( p<0 01, p<0 05 ). Conclusion High semen plasma cytokines contents represent existing local infection and enhanced auto-immune status, both damaging to sperms. Infertility would be the inevitable consequence. Monitoring of changes of the cytokine contents should be a part of fertility studies.