摘要
目的 :评价静脉滴注伊曲康唑防治兔侵袭性曲霉病的价值。方法 :建立免疫抑制兔侵袭性曲霉病 (IA)模型 ,动物接种烟曲霉孢子后 (治疗实验 )或前 (预防实验 )静脉滴注伊曲康唑 (15mg·kg-1·d-1)进行治疗或预防 ,以两性霉素B(1mg·kg-1·d-1)和氟康唑 (2 0mg·kg-1·d-1)为对照 ,用酶联免疫吸附试验 (ELISA)法检测连续采集的兔血清标本中的半乳甘露聚糖(GM) ,结合累积病死率、动物组织真菌负荷量对治疗和预防效果进行评价。结果 :与未治疗对照组相比 ,静脉滴注伊曲康唑组和两性霉素B组均能降低血清GM含量和组织真菌负荷量 ,两组间差异无显著性 (P >0 .0 5 ) ,但氟康唑组无显著降低血清GM含量和动物组织真菌负荷量。预防性静脉滴注伊曲康唑组其血清GM含量和组织真菌负荷量比对照组明显降低。结论 :静脉滴注伊曲康唑有显著防治兔IA的作用。
Objective: To investigate the efficacy of intravenous itraconazole in the treatment and prophylaxis of invasive aspergillosis. Methods:An immunosuppressed rabbit model of invasive aspergillosis was established. Itraconazole was given intravenously at a dosage of 15 mg/kg daily before or after challenge with Aspergillus fumigatus. The efficacy of itraconazole was compared with that of amphotericin B(1 mg·kg -1 ·d -1 ) and fluconazole (20 mg·kg -1 ·d -1 ). Serum galactomannan (GM) detected by enzyme-linked immunosorbent assay, cumulative mortality as well as fungal CFU in tissues were used for the evaluation of therapeutic and prophylactic efficacy. Results:In both itraconzole and amphotericin B groups(but not fluconazole), serum GM index and fungal CFU in tissues were markedly reduced. Serum GM index and fungal CFU in tissues were also markedly reduced in prophylactic itraconzole group. Conclusions:Intravenous itraconazole is effective in the treatment and prophylaxis of experimental invasive aspergillosis.
出处
《中国抗感染化疗杂志》
2004年第2期82-85,共4页
Chinese Journal of Infection and Chemotherapy
