细胞核转录因子和血管内皮细胞生长因子在胃癌组织中的表达及其意义

Expression and clinical significance of nuclear factor and vascular endothelial growth factor in human gastric carcinoma tissue

目的 研究早期、中期、晚期胃癌和不典型增生及慢性胃炎中细胞核转录因子 κBp65 (NF κBp65 )、血管内皮细胞生长因子 (VEGF)的表达 ,探讨胃癌发生、浸润和转移过程中NF κBp65、VEGF的表达和微血管密度 (MVD)的关系及其临床意义。方法 收集 1997年～ 2 0 0 2年期间经术后病理证实的早期、中期和晚期胃癌患者 62例作为研究对象 ,同时将 15例不典型增生及 2 0例慢性胃炎患者作为对照 ,进行免疫组织化学染色 ,检测NF κBp65、VEGF的表达和MVD ,分析它们之间的关系及其意义。结果 胃癌组中NF κBp65、VEGF的表达阳性率分别为 62 .9%、5 1.6% ,而对照组的NF κBp65、VEGF的表达阳性率仅为 17.1%、11.4% ,胃癌组与对照组NF κBp65、VEGF的表达阳性率差异有显著性 (均为P <0 .0 1)。胃癌组中的MVD明显增高 (P <0 .0 1) ;胃癌组中 ,早期、中期和晚期胃癌之间NF κBp65、VEGF表达阳性率和MVD差异有显著性 (均为P <0 .0 5 )。NF κBp65表达与VEGF表达呈正相关 (r =0 .5 93 ,P <0 .0 0 1)。胃癌组中有淋巴结转移者VEGF的表达和MVD明显高于无淋巴结转移者 (均为P <0 .0 5 )。结论 胃癌中NF κBp65、VEGF表达阳性率和MVD均增高 ,与肿瘤的浸润程度、淋巴结转移密切相关 ,NF κB。

Objective To investigate the expression of neclear factor?κBp65(NF?κBp65),vascular endothelia growth factor(VEGF)and microvessel density(MVD) in human gastric carcinoma,and to study the relationship among expression of NF?κBp65,VEGF and MVD and their significance in human gastric carcinoma.Methods Sixty two patients with gastric carcinoma who underwent surgical resection from 1997 to 2002 were followed up .Expression of NF?κBp65,VEGF and MVD were examined by S?P immunohistochemistry in 62 archival paraffin embedded gastric carcinoma tissue specimens and 35 patients of control.The relationship among expression of NF?κBp65,VEGF and MVD in human gastric carcinoma and control group were analyzed by SPSS 10.0 version software.Results The expression of NF?κBp65,VEGF were obviously higher in gastric carcinoma group than that in the control group(62.9% versus 17.1%,51.6% versus 11.4%,respectively,all P <0.001).The difference between expression of NF?κBp65、VEGF and MVD in different stages of gastric carcinoma were significant(all P <0.05).The expression of NF?κBp65 was significantly correlated with VEGF( r =0.593, P <0.001).The expression of VEGF and MVD were significantly higher in lymph node metastases group than that in no lymph node metastases group(all P <0.05).The expression of NF?κBp65 and VEGF were highly correlated with MVD ,which was significantly higher in the NF?κBp65(+) group and VEGF (+) group(all P <0.001).Conclusion The expression of NF?κBp65,VEGF and MVD are associated with the different stages of gastric carcinoma,lymph node metastases and prognose.Expression of NF?κBp65and VEGF are highly correlated with MVD.The relationship among NF?κBp65,VEGF and MVD indicates that NF?κBp65,VEGF may play an important role in occurrence and metastases of gastric carcinoma.