骨髓间充质干细胞移植对小型猪急性心肌梗死模型治疗作用的初步研究

Primary study of self-transplantation of bone marrow mesenchymal stem cell for pigs with acute myocardial infarction

目的 观察自体骨髓间充质干细胞 (MSC)心肌内移植对小型猪急性心肌梗死 (AMI)的治疗效果。方法 30只小型猪制备AMI模型 ,实验组 2 2只取骨髓分离MSC ,体外培养扩增后注入至缺血坏死心肌周围 ,对照组 8只输注等量生理盐水 ,1个月后比较两组的生存率、存活心肌来源和功能性蛋白质的表达。结果 实验组存活率 10 0 % ,存活心肌增加并检测到 5 溴脱氧尿嘧啶 (Br dU)标记物 ,心肌钙蛋白Ⅰ (cTnⅠ )等心肌功能性蛋白质的表达。结论 AMI小型猪自体骨髓MSC心肌内移植后 ,梗死区出现MSC来源的新生心肌 。

Objective To study the therapeutic effect of bone marrow mesenchymal stem cell(MSC) transplantation for pigs of acute myocardial infarction.Methods Twenty pigs of 30 kg weight were made of acute myocardial infarction models which were divided into two groups randomly.In the therapeutic group,30 ml marrow were aspirated and MSC were islolated and cultured in vitro.Subsequently the MSCs were collected and transplanted to the ischemic zone of the hearts while in control group only 0 9% sodium were injected in the same way.Newly generated cardiomyocytes were determined and cardiac special proteins of cardiac troponin Ⅰ etc were also tested by immunohistochemistry.Results All the hearts of MSC transplanted pigs were found that newly generated cardiac cells were positive of BrdU,proved that the new cardiac cells were differentiated from MSCs.Cardiac troponin Ⅰ was also determined positive in these new cardiac cells.Conclusion Self-transplantation of MSCs induced newly generated cardiac cells in hearts of acute myocardial infarction pigs.