白细胞介素-1β对谷氨酸钠致痫大鼠海马Gs蛋白表达的影响

THE EFFECTS OF INTERLEUKIN-1 BETA ON THE EXPRESSION OF Gsα IN HIPPOCAMPUS OF EPILEPTIC RATS INDUCED BY SODIUM GLUTAMATE

目的 探讨白细胞介素 1β(Interleukin 1,IL 1β)在谷氨酸钠致痫大鼠中对海马兴奋性G 蛋白α亚基(stimulatedG proteinαsubunit,Gsα)蛋白表达的影响 ,为阐明IL 1β在致痫中的作用机制提供线索。 方法 免疫组织化学方法结合行为观察 (SD大鼠随机分为对照组、GluNa组、IL 1β +GluNa组、rhIL lra+IL1β +GluNa组和D AP 5 +IL 1β+GluNa组 )。 结果 行为观察显示 ,IL 1β+GluNa组大鼠痫性发作潜伏期 (平均 2min)较其他组 (平均 6min)明显缩短 ,且发作程度 (Ⅲ～Ⅳ级 )较其他组 (Ⅰ～Ⅲ级 )严重 ;对照组无痫性发作。免疫组织化学染色显示 ,Gsα蛋白在海马各区均有表达 ,IL 1β +GluNa组大鼠在齿状回、CA1区和CA3区Gsα表达较其他组明显增强。 结论 IL 1β参与致痫 ,且在谷氨酸致痫中可能通过Gs蛋白介导发挥作用。

Objective In order to explore the action of IL-1β involved in epileptogensis,the changes of Gsα expression were studied in hippocampus of epileptic rats induced by sodium glutamate. Methods Immunohistochemical staining and animal behaviours were observed during the experiments.Healthy adult SD rats were divided randomly into five groups:1.Control.2.GluNa group.3.IL-1β+GluNa group.4.rhIL-1ra+IL-1β+GluNa group.5.D-AP-5+IL-1β+GluNa group. Results The changes of behaviours showed that latent period of seizure in IL-1β+GluNa group was reduced remarkly compared with that of other groups;the degrees of seizure were more serious than that of other groups.The results of immuohistochemistry showed that immunoreaction of Gsα was obviously enhanced in hippocampal CA1,CA3 areas and dentate gyrus of IL-1β+GluNa group compared with other groups.Conclusion IL-1β may play an important role in medicating pathologenesis procession of epilepsy induced by glutamate and make effect by stimulating overexpression of Gs protein.