尘粒引起人支气管肺淋巴结巨噬细胞的凋亡和bcl-2表达

APOPTOSIS AND BCL-2 EXPRESSION OF DUST-LADEN MACROPHAGES OF HUMAN BRONCHOPULMONARY LYMPH NODES

目的 研究人支气管肺淋巴结尘细胞和大鼠腹膜腔巨噬细胞吞噬碳粒后的凋亡和bcl 2表达 ,探讨巨噬细胞凋亡与淋巴结结构变化之间的关系。 方法 取人支气管肺淋巴结 ,作石蜡切片和超薄切片 ,观察尘粒分布、凋亡尘细胞和组织结构变化。用TUNEL染色法和bcl 2抗体标记法观察淋巴结内尘细胞和碳粒处理后的巨噬细胞的凋亡变化和bcl 2表达。 结果 成人淋巴结巨噬细胞的尘粒明显沉积 ,淋巴组织减少 ,胶原纤维增生 ,血管密度增加。在超薄切片上 ,尘细胞的细胞核固缩 ,出现空泡。淋巴结尘细胞和巨噬细胞吞噬碳粒后 2 4h都出现TUNEL染色和bcl 2抗体标记阳性细胞。分解尘粒和碳粒活跃的巨噬细胞 ,bcl 2表达特别明显。 结论 尘粒沉积引起人支气管肺淋巴结巨噬细胞的凋亡和凋亡抑制基因bcl 2高表达 ,尘细胞凋亡与成人支气管肺淋巴结的结构变化有关。

Objective To investigate apoptosis and bcl-2 expression of dust cells in human bronchopulmonary lymph nodes and murine peritoneal macrophages treated with carbon particles and study the relation of apoptosis of dust-laden macrophages and structural changes of the lymph nodes. Methods Distribution of dust particles, apoptotic cells and structural changes of the lymph nodes were viewed on paraffin sections and ultrathin sections. Apoptosis and bcl-2 expression of dust cells in human bronchopulmonary lymph nodes and macrophages treated with carbon particles were observed with TUNEL staining and bcl-2 antibody labeling. Results In the bronchopulmonary lymph nodes of the adult group, dust particles were deposited significantly in macrophages, the lymphatic tissue decreased, collagen fibres and density of blood vessels increased. In ultrathin section, the nucleus condensed and contained vacuoles. There were TUNEL-positive cells and bcl-2 labeling positive cells in dust cells of the lymph nodes and macrophages 24*!h after phagocytizing carbon particles. Bcl-2 was expressed strongly in the active macrophages decomposing dust particles or carbon particles. Conclusion Deposition of dust particles induces apoptosis and overexpression of antiapoptotic gene bcl-2 of macrophages in human bronchopulmonary lymph nodes. Structural changes of the bronchopulmonary lymph nodes in adult may relate to apoptosis of dust-laden macrophages.