幽门螺旋杆菌对慢性胃炎患者粘膜内D细胞及生长抑素mRNA表达的影响

THE EFFECT OF HELICOBACTER PYLORI INFECTION ON THE EXPRESSION OF D CELL AND SOMATOSTATIN mRNA IN THE PATIENTS WITH CHRONIC GASTRITIS

目的 了解幽门螺旋杆菌 (Hp)感染与慢性胃炎患者胃窦粘膜内D细胞及生长抑素mRNA+ 细胞 (DmRNA+ 细胞 )减少的关系。 方法 免疫细胞化学和原位杂交技术。 结果 正常组D细胞与Hp- 胃炎组无差别(P >0 0 5 ) ,但Hp+ 胃炎组D细胞显著减少 ,与Hp- 胃炎组相比有显著性差异 (P <0 0 1)。DmRNA+ 细胞 ,Hp+ 胃炎组也低于其他 2组且有显著性差异 (P <0 0 1)。 结论 Hp+ 胃炎组内D细胞及DmRNA+ 细胞减少与Hp感染有密切关系。它能减少D细胞分泌的生长抑素对G细胞分泌的影响 ,但是生长抑素基因的转录和蛋白的合成依然运行。

Objective To investigate the relationship between D cells and somatostatin mRNA + cells decreased in the antral mucosa of patients with chronic gastritis and helicobacter pylori infection. Methods Immunocytochemistry and in situ hybridization. Results The results indicated that the number of D cells was not obvious different between the control and HP+- groups(P>0.05), but in the HP + group, the D cells markedly reduced as compared with HP - group and showed a significantly difference(P<0.01). Somatostatin mRNA + cells in the HP + gastritis group was also lower than that of the other two groups, and had a significant difference (P<0.01).Conclusion The D cells and somatostatin mRNA + cells decreased in the HP + group closely related to the Hp infection, which can be able to reduce the inhibition of G cell's secretion by D cell producing somatostatin, but the transcription of somatostatin gene and protein synthesis were runing well.