摘要
我们应用放射免疫测定法观察了18例烧伤病人血浆β-内啡肽免疫活性物质(ir-βEP)的动态变化,实验结果表明:烧伤后3天内各期血浆 ir-βEP 均显著高于正常对照值,并于伤后4～5天恢复正常,同时,烧伤病人自体血浆参与的淋巴细胞体外培养亦表明,淋巴细胞对 PHA 的反应性在伤后3天内明显抑制,于伤后4～5天恢复正常.而经抗βEP 血清处理后的自体血浆淋巴细胞培养对 PHA 的反应性较抗βEP 血清处理前有明显的增强。实验结果提示:烧伤作为一个强烈的应激性刺激可引起血浆 ir-βEP 的升高;血浆 ir-βEP 能够抑制淋巴细胞对 PHA 的反应性。由此推断,烧伤早期血浆 ir-BEP 的升高是导致烧伤后免疫抑制的重要原因之一.
The study of immunosuppression after thermal injury has aroused great interest.The pre- sent study is an investigation of changes of plasma ir-βEP in patients and their effect on lymphocyte responses to PHA following thermal injury,in order to establish a relationship between the immune and neuroendocrine system. Plasma ir-βEP in eighteen burn patients was determined using the radioimmunoassay. At the same time,autoplasma and the autoplasma treated with anti-βEP serum had been both tested for their effect on lymphocyte responses to PHA. The levels of plasma ir-βEP were found to be elevated significantly in every phases du- ring the first three days postbarn(P<0.0005-P<0.001),and then declined to the normal level on the fourth or fifth day postburn.The lymphocytes cultured with autoplasma showed their inhibited responses to PHA.daring the first three days postburn and returned to normal on the fourth or fifth day postburn,while the autoplasma treated with anti-βEP serum had an effect to improve the lymphocyte responses to PHA. The findings suggest that burn stress is a strong stimulation which may elevate plasma ir-βEP and whieh in turn decrease the lymphocyte response to PHA.We assume that increased plasma ir-βEP may be one of the main causes of immunosuppression after thermal injury.
