虎杖中主要蒽醌的提取分离及HPLC分析测定

Extraction,Separation, HPLC Analysis of the Main Anthraquinones in Polygonum cuspidatum

改进了碱梯度萃取虎杖中重要组分蒽醌的分离提取方法 ,在碱梯度萃取大黄素过程中 ,采用少量多次提取和分段收取方法 ,可获得大黄素产品而不夹带大黄素甲醚 ,可连续大规模提取分离大黄素和大黄素甲醚 ,提取率大于 70 % ,产品纯度大于 97%。改进了文献报道HPLC测定虎杖中单一大黄素的方法 ,改换C8柱和改变检测波长 。

The extraction and separation processes of the main anthraquinones in Polygonum Cuspidatum, including emodin, rhein and physcion, have been carried out and improved. More pure emodin products without physcion could be obtained from a small amount and by repetitive extraction and stepwise collection. The extraction yields of emodin and physcion were more than 70%, with purity greater than 96%. The HPLC analysis method of anthraquinones was improved by using C18 column instead of C8 column, with detection wave-length of 437 nm. The method could afford to simutanniously determine the main anthraquinones in Polygonum Cuspidatum.