轻稀土化合物抑癌作用及机制的研究

Study on the anti-tumor effect and mechanism of light thulium compound

目的 在探讨稀土与肿瘤关系的基础上进一步证明稀土的抑癌作用及探讨作用机制 ,最终的目标是为稀土在医药保健事业方面开创一个新领域。提供确定、可靠的科学依据。方法 通过动物实验及细胞培养 ,采用生化、免疫、分子生物学等方面的规范方法及高新技术的应用 ,如用电子顺磁共振 (FSR)自旋扑获技术测定自由基、Northern杂交技术分析稀土元素对抑癌基因的表达等。此外 ,还对接触稀土人群进行了 10年的动态观察和肿瘤流行病学回顾性的队列调查等。结果 ①整体抑癌实验 :按卫生部颁发的药效实验规程 ,给小鼠腋下种瘤株 ,腹腔注射ReNO3 、ReCl3 等 ,剂量在 3 2及 64mg/kg时对S180 肉瘤生长的抑制率分别为 3 9 87%及 2 7 3 4%、Lewis肺癌生长抑制率分别为 3 6 82 %及 2 9 5 5 %均有明显的统计学意义。给AMAE小鼠腹腔注射氨基甲酸乙酯的肺癌剂同时饮用ReCl3 (0 0 62 5 %、0 2 5 %、1 0 % ) 12 0d时其肿瘤发生率分别为 75 %、61 8%、79 6% ,而未给稀土组为 90 0 % ,表明稀土对化学致肺腺癌小鼠亦具有明显抑制作用。②体外抑癌试验 :对人白血病细胞 (K562 )在ReNO3 浓度为 0 0 3 2～ 0 .5mg/ml时 ,生长的抑制率为 15 8%～ 3 4% ,在ReCl3 浓度为 0 0 1～ 0 .5mmol/L时其细胞生长抑制率为 18%～ 68% ,而?

To study the anti-tumor effect and mechanism of light thulium compound.Northern blot analysis and retrospective cohort survey were carried out. 1.Inhibition of cancer growth in vivo showed: when the transplanted tumor model was injected with ReNO3 and ReCl3 at the dose of 32 and 64 mg/kg, the inhibition rate were 39.87% and 27.34% respectively. The inhibition rate for Lewis lung cancer was 36.82% and 29.55%. When the AMAE mice were injected with carbaminate and RECL3 (0.062 5%, 0.25% and 1.0%) for 120 days, the incidence of lung cancer were 75%, 61.8% and 79.6% respectively. That of the contrast group was 90.0%. 2. Inhibition of cancer cell growth in vitro showed: when h2~0.uman leukemia cells (K562) were treated with ReNO3 with the density of 0.032～0.5 mg/ml, the inhibition rate was 15.8%～34%. 3. Study on the mechanism of inhibition on cancer showed: when human gastric cancer cell line were treated with 1.5 mmol/L lacl3 or 1.75 mmol/L CaCl 3 (Recl 3), its P 53 andP 16 genes expression increased obviously. 4. Retrospective cohort study on the tumor showed: the mortality of RE group and the contrast group were 19.675 2/105 and 87.948 4/105 respectively, RR was 0.223 7 (P<0.05). Death rates of cancer cells were 0/10 5 and 40.923 0/10 5 respectively (P<0.05).[Conclusion]From microcosm to macrocosm, from animal test to crowd investigation, all the results proved that the light thulium has inhibitive function on cancer.