摘要
用不同剂量、剂量率的6 0 Coγ射线对卵黄液中的EDS 76病毒进行辐照 ,研究了其对病毒的灭活效果和对卵黄抗体 (IgY)的影响。结果表明 ,6 0 Coγ射线辐照 ,可以灭活高免卵黄液中的EDS 76病毒 ,且随辐照剂量、剂量率的增大 ,灭活率也增高 ;EDS 76病毒在卵黄液中的D10 值为 0 .57kGy～ 0 .60kGy ;6.0kGy的辐照剂量 ,可以将高免卵黄液中的EDS 76病毒完全灭活 ,且不影响卵黄抗体效价 ,该卵黄抗体稳定性好、耐酸、耐热。
EDS 76 virus in the yolk were irradiated using different dosage, dosage rate of 60 Coγ,and the inactivation effect and the effect on yolk antibody (IgY)were studied. The results showed that EDS 76 virus could be killed and the inactivation rate increased with the increase of irradiation dosage and dosage rate . The D 10 value of EDS 76 virus in the yolk was 0.57～0.60kGy; EDS 76 virus could be totally inactivated by the dosage of 6.0kGy, and the antibody titre still didn't change and showed good resistance against heat, acid and trypsase.
出处
《激光生物学报》
CAS
CSCD
2004年第2期111-115,共5页
Acta Laser Biology Sinica
基金
山西省科委攻关项目 ( 9710 15)
