3,6—二—[二甲氨基]二苯并碘六环镧乙二胺四乙酸盐配合物对L_(7712)细胞DNA,RNA和蛋白质合成的影响

Effects of Complex of 3,6-Di-(Dimethylamino)-Dibenzopyriodonium Lanthanum(Rare Earths)EDTA on Syntheses of DNA,RNA and Protein in Leukemia 7712 Cells in Vitro

3,6-二-[二甲氨基]二苯并碘六环镧乙二胺四乙酸盐配合物对 L_(7712)细胞 DNA 合成的 ID_(50)为12μg/ml,对 L_(7712)细胞 DNA,RNA 和蛋白质代谢动态过程的抑制作用,都随时间的延长而增加.对 DNA 合成的抑制作用,似属模板损伤型.

Complex of 3,6-di-(dimethylamino)-dibenzopyriodonium lanthanumEDTA(32μg/ml)inhibited the incorporations of [~3H]thymidine,[~3H]ur-idineand[~3H]leucine into DNA,RNA and protein of leukemia 7712 cellsduring the period of 3-24h after exposure in vitro.The degree of inhibi-tion increased with the incubation time.ID_(50) of 3,6-di-(dimethylamino)-di-benzopyriodonium lanthanum EDTA on DNA synthesis in leukemia7712 cells at 24h was 12μg/ml The rate of DNA synthesis decreased withtime.The mechanism of inhibition was likely due to damage of DNAtemplet.