摘要
改进筛选新生隐球菌Cap5 9荚膜缺陷株ura5突变株的方法。采用硫酸二乙酯化学诱导新生隐球菌Cap5 9荚膜缺陷株 ,利用 5 氟乳清酸 (5 FOA)反筛选法筛选ura5尿嘧啶合成基因突变株。用新方法筛选到 2株Cap5 9荚膜缺陷株ura5突变株。
This study was to improve the way for selecting ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strains.They were induced by Diethyl Sulfate. Ura5 mutants were screened by 5-fluoroorotic acid counter selection method. Using the new method, we obtained two ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strain.A easy method that was used to screen ura5 mutants of Cryptoccocus neoformans has been established.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第2期105-107,共3页
Microbiology China
基金
国家自然科学基金资助项目 (No 395 70 0 4 3)
