湿地松种子衬比射线检验方法的研究

X-RAY CONTRAST METHOD FOR TESTING GERMINABILITY OF SLASH PINE(Pinus elliottii) SEEDS

对温地松种子进行对比射线方法检验,结果表明,其最佳x射线摄影衬比过程为:将种子在25℃下浸种24h,擦干种子表面水分后,置入40%KI溶液中处理5h,在流水中冲洗5～6min,置于70℃下干燥24h。使用SOⅡ胶片和HY—35型射线机时,拍摄条件为:电压20kV,电流4mA,曝光时间100s,焦片距25cm。由此认为,未被浸渗而胚和胚乳发育良好的种子具生活力,可用来估算种子的发芽能力。用上述衬比方法测定35份湿地松种子,其发芽能力与实际发芽率相当一致。

X-ray contrast method of slash pine seeds has been proposed as follows: The seeds are soaked in tap water at 25℃ for 24 hours,. dried superficially and steeped in 40% aqueous solution of KI for 5 hours. After superficially washing in slowly running water for 5-6 minutes, the seeds are dried at 70℃ for 24 hours. The dried seeds are radiographed under the following conditions: kV=20, mA=4, exposure time=100 seconds, focus-film-distance=25cm. The unimpregnated full seed is considered as viable seed for calculating germinability. The germinability (X) of 35 seed samples is in accordance with the germination percent (Y). Their relationship is Y=-10.2+1.11X, r=0.98.