α-半乳糖苷酶酶解B型长臂猿细胞回输A型长臂猿的研究

B Gibbon Red Blood Cells Digested by α-Galactosidase Transfused to A Gibbon

使用基因重组咖啡豆α 半乳糖苷酶体外处理B型长臂猿红细胞 ,使其转变为O型 ,再回输给A型长臂猿 .α 半乳糖苷酶可以清除B型长臂猿红细胞表面B抗原 ,而不影响红细胞结构、功能及其在受体体内存活 .α 半乳糖苷酶酶解的B型红细胞输给A型血的长臂猿 ,未发生输血反应 ,受血猿的血液及尿液常规指标与输血前相比 ,无明显变化 .

In order to perform the animal transfusion experiment before entering clinical trial of B→O blood conversion,B type of gibbon red blood cells were digested by recombinant coffee bean α galactosidase which resulted in B→O blood conversion.The converted red blood cells were transfused into the recipient bibbon with A blood type.The results showed that α\|galactosidase could remove blood B antigen located on the surface of gibbon red blood cells efficiently.There were no harmful effects on donor red blood cell structure and function as well as on donor cell survival in recipient gibbon after α\|galactosidase digestion.The results also demonstrated that no transfusion rejection happened in recipient gibbon with different blood group.The routine blood and urine tests of recipient gibbon were normal after enzymatically converted blood cell transfusion.