摘要
To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2004年第2期275-279,共5页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家转基因植物研究与产业化专项 (No :J0 0 B 0 0 3 2 )资助~~
