摘要
目的:探讨乙型肝炎病毒(HBV)基因组是否存在前-前-S编码基因,并探讨前-前-S基因与HBV基因型分布之间的关系. 方法:自慢性乙型肝炎患者血清中提取病毒基因组DNA, 首先应用多引物-多聚酶链反应(PCR)进行HBV基因型分型,之后将前-前-S区扩增,TA克隆到pGEM Teasy载体后进行单克隆测序,利用Vector 8.0软件进行序列分析. 结果:选择15例患者,经过HBV基因型分型实验确定基因型为B型者1例,B/C混合型者5例,C型9例.自15例患者血清中提取的HBV基因组中扩增出前-前-S基因片段,TA克隆后选择31个克隆进行测序,测序结果证明这31个克隆均编码前-前-S基因. 结论:前-前-S区编码在B、C基因型中均为普遍存在的现象.
AIM: To try to identify pre-pre-S region in hepatitis B virus (HBV) genome and to study the relationship between the genotype and the existence of pre-pre-S region. METHODS: Genomic DNA of HBV was extracted from the sera of patients with HBV infection. Multiple-primers poly-merase chain reaction (PCR) method was used to identify the genotype of HBV strains. Then the pre-pre-S region was amplified by PCR method, and TA cloned into pGEM Teasy vector. After sequencing of the target region, vector 8.0 software was used to analyse the sequences. RESULTS: Fifteen male patients were collected in this study. There were 1 genotype B, 5 genotype B and C mixture, and 9 genotype C. Amplified PCR products of the pre-pre-S region from these 15 samples were ligated into the pGEM Teasy vectors. After sequencing, 31 clones were found being encoding for pre-pre-S peptide. CONCLUSION: Existence of coding gene of the pre-pre-S region is popular in B or C genotype of HBV.
出处
《世界华人消化杂志》
CAS
2004年第4期785-789,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金攻关项目
No.C03011402
No.C30070689军队"九
五"科技攻关项目
No.98D063军队回国留学人员启动基金项目
No.98H038军队"十
五"科技攻关青年基金项目
No.01Q138军队"十
五"科技攻关面上项目
No.01MB135~~
