pAW101-EcoRⅠ限制性片段在广东人群中的分布及其在法医学上的应用

DNA RFLPS DETECTED BY pAW101 PROBE IN THE POPULATION OF GUANGDONG; CHINA

用pAW1015kb的片段为探什,检测广东地区106例无血缘关系居民的EcoRI酶切限制性片段(RF)的分布情况。共发现长度各异的65条RF,每人只具有其中的1～2条。统计学分析证明,这些RF的分布符合哈一温氏平衡。无血缘关系的两个个体间具有完全相同的RF图谱的机会为0.00048(相当于l/2080)。家系调查结果表明,这些RF符合孟德尔遗传规律。本文是应用作者推导出来的计算RF大小的数学模式。此法在作亲权鉴定时,父权排除率可达0.914,相当于目前国内所用的血型测定法(包括HLA-A,-B抗原测定)所达到的总和,说明此方法在法医学个人识别和亲权鉴定中具有广阔的应用前景。

The distribution of EcoRI restriction fragments (RFs) detected by pAW101 probe in the population of Guangdong, China, were reported. 65 independent fragments among 106 unrelated individuals tested were observed. Each person bears one or two fragments only. The distribution of occurrence frequencies of 65 fragments is consistent with Hardy-Weinberg equilibrium. The probability bearing complete homologous polymorphism is 0.00048. The inheritance pattern of these RFs shown by family examination is consistent with Mendelian Law. This paper used a mathematical model induced by authors for calculating the sizes of RFs. The exclusion probability of paternity is 0.914, which is equal to the level obtained by the blood typing (including HLA-A, -B typing) currently used in China. It shows that this method has a potential for wide application to paternity testing and to individual-identification.