人类自然杀伤细胞对靶细胞的选择性结合

SELECTIVE ACTION OF HUMAN NK CELLS ONALLOGENEIC TARGET CELLS

采用单个细胞水平细胞毒方法研究比较了正常人外周血自然杀伤(NK)细胞对不同靶细胞结合与杀伤活性的差别。发现NK细胞对人胚胎成纤维细胞和低分化鼻咽癌(CNE-2)细胞的结合率明显高于对人成骨肉瘤细胞(OS-732)和PLC/PRF/5人肝癌细胞,结合并杀伤新鲜切除人肝癌细胞,而对同一病人的癌周肝细胞则无作用;在同一株CNE-2细胞中,NK多选择性地与核浆比例大的靶细胞结合。以上结果说明NK细胞在发挥自然细胞毒过程中具有一定的选择性,选择性地结合并杀伤分化差或幼稚的靶细胞。

Selectivity of human natural killer (NK) cells, separated with nylon wool columns from human peripheral blood, on the binding and killing actions on different cytological types of target cells were investigated at the single cell level. It was found that undiffer-entiated nasopharyngeal carcinoma cells (CNE-2) and human fetal fibroblast cells were liable to be attached more than human osteosarcoma (OS-732) and human hepatoma cells (PLC/PRF/5). NK cells killed neoplastic hapatocytes but neglected autologous non-neoplastic hepatocytes of the same patients. Furthermore, the cells with poor differentiation and high nucleus/cytoplasm ratio in CNE-2 cell line were easily bound by NK cells. These results suggest that NK cells can selectively recognize and conjugate poorly differentiated or immature target cells.