Ⅱ型登革病毒对人骨髓造血细胞抑制机制的初步探讨

PRELIMINERYRESEACH ON THE INHIBITORY MECHANISM OF DENGUE-2 VIRUS ON HUMAN CFU-GM AND CFU-MK IN VITRO CELL CULTURE

用Ⅱ型登革病毒(D_2 V)与人骨髓细胞混合培养48小时后,测定上清液中干扰素的浓度,发现D_2 V感染组的干扰素浓度明显高于空白对照组(P<0.001)。在骨髓CFU-GM培养中,加入D_2 V及抗γ-干扰素后,CFU-GM集落产率明显高于只加D_2 V而不加抗γ-干扰素组(P<O.001).在去除骨髓T细胞后作CFU-MK培养,结果显示D_2 V感染组与空白对照组的CFU-MK集落产率无显著差异(P>0.05)。实验研究结果提示D_2 V对人骨髓的抑制与免疫及体液因素有关。

The concentration of interferon detected in the supernatant fluid of Dengue-2 virus (D2V) in human bone marrow cell culture cultured 48 hours was significantly higher than the control group(P<0.001). When anti γ-interferon was added to CFU-GM containing D2V and γ-interferon to counteract the interferon, the plating efficiency of CFU -GM was significantly higher than in the specimen with no anti γ-interferon added (P <0.001). There was no significant difference between plating efficiency of CFU-MK containing D2V and normal control when bone marrow T cell were removed before the test. Our experiment suggests the inhibition of bone marrow by D2V has a relation to immunity and humoral factors.