含锌煅烧牛松质骨复合骨髓基质细胞构建组织工程骨

Tissue engineered bone constructed by compounding zinc-sintered bovine cancellous bone with marrow stromal cells

目的 探讨含锌煅烧牛松质骨和单纯煅烧牛松质骨复合骨髓基质细胞 (MSCs)构建的组织工程骨在体内的成骨能力。方法诱导培养骨髓基质细胞 ,将其分别接种至含锌煅烧牛松质骨及单纯煅烧牛松质骨 ,植入肌肉内 ,分别于术后 4、8周处死实验动物 5只 ,行组织学观察和新生骨面积测定。结果 术后不同时间组织学观察含锌组新生骨明显多于单纯组 ,新生骨面积测定值含锌组分别为 (17619.14 5± 44 5 9.816)、(2 5 2 3 8.5 3 9± 1995 .164 ) μm2 ,单纯组分别为(80 5 6.5 3 7± 5 67.60 3 )、(10 787.95 7± 92 5 .5 0 9) μm2 。含锌组明显大于单纯组 (P <0 .0 5 )。结论 含锌煅烧骨作为骨组织工程的支架材料优于单纯煅烧骨 ,是一种理想的生物活性支架材料。

Objective To study the osteogenic ability of tissue engineered bone constructed by compounding both the zinc-sintered bovine cancellous bone and the sintered bovine cancellous bone with marrow stromal cells (MSCs) in vivo respectively.Methods The MSCs were induced by dexamethasone,vitamin C,and β-glycerophosphate,and then they were seeded into the zinc-sintered bone and sintered bone respectively.Each of the tissue engineered bone was implanted into the muscles of 10 experimental animals.The animals in the two groups were killed.The newly-formed bones were observed histologically and the areas of new osseous tissues measured at the 4th and 8th week after the implantation.Results The osteogenic capability was confirmed by alkaline phosphatase staining,calcification nod chinalizarin staining and economycin fluorescence staining.At different postoperative time points,the areas of the new osseous tissue of the zinc- sintered bone,which were (17?619.145±4?459.816) μm 2 at the 4th week and (25?238.539±1?995.164) μm 2 at the 8th week,were significantly ( P <0.05) larger than those of the new osseous tissue of the sintered bone,which were (8?056.537±567.603) μm 2 and (10?787.957±925.509) μm 2 respectively.Conclusion The zinc-sintered bovine cancellous bone,which can produce more satisfactory results than the sintered bone in performing as the scaffold in bone tissue engineering,is an ideal bio-active scaffold.