壳聚糖神经干细胞复合物修复大鼠完全性脊髓损伤的组织学观察

Histologic observations of the complex of chitosan and neural stem cells after transplantation into the gap of adult SD rat spinal cord

目的 探讨壳聚糖与神经干细胞复合物修复成年SD大鼠完全性脊髓损伤的可行性。方法 制作大鼠脊髓完全缺损模型 ,在形成的缺损中植入壳聚糖与神经干细胞复合物 (实验组 ) ,或只植入壳聚糖 (对照组 ) ,或不加任何材料 (空白组 ) ,术后 2、4、8周灌注取材 ,观察结果。结果 壳聚糖与神经干细胞复合物在植入SD大鼠脊髓完全缺损模型后 ,能桥接缺损两端脊髓 ;其内的神经干细胞能存活、迁移并分化成多种形态的神经组织细胞 ,并诱导神经轴突向复合材料内生长。结论 壳聚糖与神经干细胞复合物有可能桥接并修复大鼠脊髓完全缺损性损伤。

Objective To explore the possibilities of transplantation of the complex of chitosan and neural stem cells (NSCs) in repair of complete spinal cord injury.Methods NSCs derived from the cortices of embryonic 16-day SD rat were cultured and concentrated in vitro.Adult female SD rat spinal cords were transected at T8 cord level and a 4-5 mm segment caudal to the transection was removed,and then the complex of chitosan and NSCs was positioned into the gap between the rostral and caudal stumps of the spinal cord.These rats were sacrificed at the postoperative 2nd,4th,8th week,and the spinal cord regions rostral and caudal to the graft and the graft itself were prepared for light and electron microscopy,immunohistochemistry,to inspect the growth,proliferation,migration and differentiation of these NSCs,their influence on the propriospinal neural cells and the regeneration of axon,and the change of chitosan.Results When transplanted into spinal gaps,grafts made of chitosan and NSCs could bridge the spinal gaps in appearance.NSCs in the bridge could survive,migrate and differentiate,and axons could extend into the grafts.Conclusion The complex of chitosan and NSCs can bridge the gap of spinal cord of adult SD rat.