雌激素对前列腺增生症移行区细胞Bcl-2、Bax和c-myc基因表达的影响

Effects of estrogen on the expression of Bcl-2,Bax and c-myc genes in the cells from transitional zone of benign prostatic hyperplasia

目的 探讨大剂量外源性雌激素对良性前列腺增生症 (BPH )移行区 (TZ)细胞Bcl 2、Bax和c myc基因表达的影响 ,籍此了解雌激素对BPH的治疗机制。 方法 对 3 0例经服用已烯雌酚的BPH患者 (处理组 )和 3 0例空白对照患者 (对照组 )的标本 ,采用免疫流式细胞光度术检测上述 3种基因在TZ的标记率和表达量。结果 雌激素处理后 ,TZ细胞Bcl 2的标记率和表达量分别由对照组的 2 5 .5 0 %和 73 .0 0降低为 13 .3 5 %和 44 .46(P <0 .0 1) ,Bax的表达量则由对照组的48.0 0升高为 5 6.97(P <0 .0 1)。结论 大剂量雌激素对BPH治疗作用可能与其抑制TZ细胞Bcl 2的表达和促进Bax的表达进而提高细胞凋亡率有关。

Objective To investigate the effects of estrogen on the expression of Bcl-2,Bax and c-myc genes in the cells from transitional zone (TZ) of benign prostatic hyperplasia (BPH).Methods The marked rate and expression levels of Bcl-2,Bax and c-myc genes in TZ of prostate were examined in 30 samples of patients with BPH taking estrogen and in 30 samples from patients with BPH not taking estrogen with the use of flow cytometry (FCM).Results The labeled rate and expression level of Bcl-2 in the TZ were significantly decreased from 25.50% and 73.00 to 13.35% and 44.46 respectively under the effect of estrogen ( P <0.01),while the expression level of Bax increased from 48.00 to 56.97 ( P <0.01).Conclusion The therapeutic effects of high dose of estrogen might be related with the inhibitory expression of Bcl-2,and promoting expression of Bax in TZ.