吉兰-巴雷综合征相关空肠弯曲菌的蛋白质谱特征分析

Preliminary analysis on the proteomic feature of Guillain--Barré syndrome--associated Campylobacter jejuni

目的 分析吉兰-巴雷综合征(GBS)相关空肠弯曲菌(Cj)与非GBS相关Cj的蛋白质谱特征。方法 利用双向电泳对这两类Cj各8株菌的全菌蛋白进行分离,比较蛋白质谱之间的差异,并对差异蛋白进行基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF)分析。结果 比较发现20个差异蛋白,质谱分析鉴定出17个蛋白质,包括wlaX蛋白,参与能量代谢的苹果酸脱氢酶、磷酸丙糖异构酶、镍-铁还原酶小亚单位、半胱氨酸合成酶及支链氨基酸氨基转移酶,参与细胞加工处理过程的热休克蛋白、铁吸收ABC转运系统周质铁结合蛋白、烷基过氧化氢还原酶以及与细胞表面结构有关的鞭毛蛋白、UDP-N-乙酰烯醇式丙酮酸葡萄糖胺还原酶等。结论 wlaX蛋白可能与致GBS相关脂多糖的独特结构合成或细菌的毒力有关,wlaX蛋白及鞭毛蛋白有可能为GBS相关Cj的特征蛋白。

Objective To search the marker proteins of Guillain--Barre syndrome (GBS)--associated Campylobacter jejuni (C. jejuni) by comparing the protein maps of GBS--associated C. jejuni strains with that of non--GBS--associated C. jejuni strains. Methods The whole--cell proteins of eight GBS--associated and eight non--GBS--associated C. jejuni strains were separated using the two--dimensional gel electrophoresis respectively. The differentially expressed proteins between the two sets of strains were identified by matrix- assisted laser desorption/ionization--time of flight mass spectrometry (MALDI--TOF--MS) after in--gel tryptic digestion. Results Teenty differentially expressed spots were found with seventeen identified ones using MSCOT database. These proteins were identified as wlaX protein and some other proteins involving in energy metablism (malate dehydrogenase, triosephosphate isomerase, Ni/Fe--hydrogenase small chain, cysteine synthase, branched--chain amino acid aminotransferase), cell process (heat shock protein, iron- uptake ABC transport system periplasmic iron--binding protein, alkyl hydroperoxide reductase), cell envolope (flagellin, UDP--N--acetylenolpyruvoylglucosamine reductase) etc. Conclusion WlaX proteins were probably associated with LPS biosynthesis or virulence of C. jejuni. WlaX protein and flagellin protein were the possible marker--proteins of GBS--associated C. jejuni strains.