大鼠脊髓损伤致截瘫后肠道细菌移位的实验研究

Study of bacterial translocation from gut after paraplegia caused by spinal cord injury in rats

目的:探讨大鼠脊髓损伤致截瘫后是否发生肠道细菌移位。方法:建立大鼠脊髓损伤性截瘫模型,以脊髓损伤性截瘫后12h、24h、48h大白鼠为实验组,未损伤脊髓的正常大白鼠为对照组。在无菌条件下,采集动物下腔静脉血进行内毒素定量测定和细菌培养,采集肝、脾、肠系膜淋巴结、肠腔内容物作细菌培养并进行菌种鉴定。取实验组和对照组各动物的肝、脾、肠系膜淋巴结、空肠、回肠进行病理切片HE染色检查,取空、回肠进行电镜检查。结果:大鼠脊髓损伤致截瘫后24h开始出现内毒素血症,截瘫后48h出现细菌移位。结论:大鼠脊髓损伤致截瘫后将发生肠道细菌移位,提示脊髓损伤截瘫的病人应尽早给予抗生素治疗。

Objective:To investigate whether gut origin bacterial translocation(BT)occurs after spinal cord in-jury(SCI)with paraplegia in rats.Method:Model of paraplegia was built up by injuring spinal cord of rat.The experimental group was rats of paraplegia,the control group was normal rats.The samples were collected at12hrs,24hrs and48hrs after spinal cord injury.Under aseptic manipulation,samples of blood,liver,spleen,mesen-teric lymph node(MLN)and intestinal contents were collected for bacterial cultures and endotoxin detection.The jejunum and ileum were observed by light and electron microscope.Result:Bacterial translocation and en-dotoxemia appeared24hrs and48hrs after spinal cord injury complicating paraplegia.Bacterial growth was found in MLN culture at24hrs post-injury and in blood,liver,spleen and mesenteric lymph node culture at48hrs post-injury with positive rates of50%,62.5%,75%and100%respectively.Translocational bacteria were mainly bacillus coli,Enterobacter cloaca,Escherichia coli,Vulgaris and Enterococcus faecalis.Conclusion:Gut ori-gin bacterial translocation will occur after spinal cord injury in rats,which indicates that antibiotics should be administrate to patients with spinal cord injury complicating paraplegia as soon as possible to prevent potential bacterial translocation.