He-Ne激光血管内照射对脑梗死患者血液流变学和细胞凋亡的影响

Effects of Intravascular Irradiation of He Ne Laser on Cerebral Infarction: Hemorrheology and Apoptosis

目的探讨He-Ne激光血管内照射(ILLLI)对脑梗死患者疗效、血液流变学性质、淋巴细胞增殖、凋亡和染色体的影响。方法脑梗死患者70例随机分成对照组和治疗组,各35例。对照组采用常规药物治疗,治疗组在常规药物治疗的基础上加用He-Ne激光血管内照射治疗,比较两组疗效和治疗前后的血液流变学指标、细胞增殖指数(MI)、细胞周期动力学指数(CKI)、姐妹染色单体交换(SCE)频率及淋巴细胞凋亡频率。结果治疗组有效率88.6%,对照组有效率65.7%,差异有非常显著意义(P<0.05)。治疗组全血黏度(低切,高切)、毛细血管血浆黏度,血沉、血沉方程K值、红细胞聚集指数、红细胞刚性指数较治疗前明显下降,红细胞变形指数较治疗前增加,治疗前后差异有显著意义(P<0.05)。治疗组患者ILLLI后淋巴细胞增殖活性高于对照组(P<0.05),CKI与对照组相比差异无显著意义(P>0.05);两组间SCE频率差异无显著意义(P>0.05);治疗组脑梗死患者的淋巴细胞凋亡率明显高于对照组(P<0.001),也明显高于治疗前(P<0.001)。结论低强度He-Ne激光血管内照射能有效改善血液流变学性质,改善微循环,增加脑血流量,促进神经功能的恢复,显著提高脑梗死患者的疗效,并能提高脑梗死患者的淋巴细胞增殖活性;可诱导发生较高的淋巴细胞凋亡率,对细胞没有致突变性。

Objective To explore the efficacy of He Ne laser intravascular irradiation on cerebral infarction and hemorrheology.To observe the effects of intravascular low level He Ne laser irradiation (ILLLI)of blood on cell proliferation,apoptosis and chromosome in lymphocyte from cerebral infarction. Methods Seventy cases with cerebral infarction were randomly divided into two groups:controp group(35 cases) treated only with common drugs and therapeutic group (35 cases)treated besides common drugs also by He Ne laser intravascular irradiation Their hemorrheology index and treatment results were observed and compared The blood lymphocytes of cerebral infarction were cultured before and after treatment. After that, the mitosis index(MI),cell kinetics index(CKI),sister chromatid exchanges (SCE) frequencies and apoptosis were determined Results The therapeutic group was better than the control one The effective rate in the therapeutic group was 88 6%, in the control one was 65 7% The viscosity and fibrinogen,etc were better than that in the control group with singnficant difference (P<0 01) The lymphocyte proliferation index was significantly two increased than the control one (P>0 05)in cerebral infarction patients after treatment; The CKI of lymphocytes had no obvious difference among groups(P>0 05);SCE frequencies of lymphocytes had no statistic significance between control group and ILLLI one (P>0 05) It showed the apoptosis rate of lIymphocytes in cerebral infarction patients after ILLLI treatment increased significantly compared with the control group, (P<0 001) While there was a significant difference of apoptosis rate of lymphocytes in cerebral infarction patients than the control(P<0 001) Conclusions During the He Ne laser intravascular irradiation of the cerebral infarction, the low level He Ne by ILLLI can increase the proliferation of lymphocytes, and can induce lymphocytes to apoptosis, but has no mutagenicity of cells