甘草次酸钠对小鼠移植性肿瘤作用的实验观察

Antitumor Effect of Na-Glycyrrhetinate on Transplanted Mouse Tumors

本文观察了甘草次酸钠对小鼠移植性肿瘤的抑制作用,并在此基础上观察了其对瘤细胞分裂指数和超微结构的影响,以及对抗肿瘤药物作用的影响。甘草次酸钠腹腔给药对小鼠S180、HepS具有不同程度的抑制作用,在10mg/kg时,抑制率分别为53.13％和40.10％(P值<0.05)。该药对HepA细胞分裂指数有抑制作用,24小时时,分裂指数抑制率为43.15％。电镜观察发现;使HepA细胞线粒体空泡变性,甚至界膜破损,细胞质中糖元颗粒减少,粗面内质网腔缩小,核染色质变细疏,膜完整性示踪剂镧颗粒进入胞质和核内。该药与抗肿瘤药物合用因不同药物和给药时间相异其增效作用亦不同,以ip喜树碱5分钟后投与本药为佳。此外本药对喜树碱引起的白细胞减少亦有保护作用。

Sodium glycyrrhetinate (GC-Na) was studied in the transplanted mouse tumors. The experiments showed. that S_(180) and Hep were significantly in- hibited by GC-Na. The inhibition rates of GC-Na, ip 10 mg/kg×10d for S_(180) and Hep in mice were 53.13%(P<0.05) and 40.10%(P<0.05). Moreover, the inhibition rate of mitotic indices of hepatoma cells were moderately in- creased affter ip administration of GC-Na with 36.81% for 1h and 43.15% after 24h (P<0.05). Electron microscopic observation: Vacuolations were found inside the cytoplasm of tumor cells. The mitoc- honderia of hepatoma cells showed s W- elling, dissolution and disapperance of cristae. The combining administrations of GC-Na with camptothecine, 5-Fu and Cis-DDP were studied. GC-Na and camptothecine at suitable interval of 5 min showed significant antitumor effect. Furthermore, GC-Na could improve leukopenia caused by campt- othecine.