鸡巨噬细胞炎性蛋白-1β基因cDNA的克隆及序列测定

Molecular clone and sequence of avian macrophage inflammatory protein-1β(MIP-1β)

为进一步研究鸡巨噬细胞炎性蛋白＿1β(MIP＿1β)的生物学功能,用反转录＿多聚酶链反应(RT＿PCR)技术,以鸡的外周血单核细胞中提取的RNA为模板,进行反转录合成和PCR扩增,获得MIP＿1β的cDNA克隆,并测得MIP＿1β的cD NA序列为273bp。其核苷酸序列与OleksiPetrenko等报道的序列有98%的同源性,氨基酸同源性为95%。

In order to research biological function of avian macrophage inflammatory protein-1βfurthermore, cDNA of MIP-1β was get by reverse transcription and PCR proliferating from avian monophages of peripheral blood. After conjonction with plasmid, planting into E.coli DH5α, cloning, the sequence of MIP-1β cDNA was get. It's homloguse is 98 % with that of reported by Oleksi Petrenko et al.