摘要
目的:探讨表皮生长因子对小鼠植入前胚胎体外发育的影响。方法:用添加不同浓度EGF(epidermal growth factor)的CZB培养液对小鼠早胚进行体外培养,观察囊胚发育率和胚胎细胞数的变化。结果:HCG(human chorionic gonadotropin)注射后138 h,0.1ng/ml EGF添加组扩张囊胚率、孵化囊胚率显著高于其他各组。100.0ng/ml EGF添加组扩张囊胚率和孵化囊胚率显著低于其余各组,且囊胚细胞死亡较多。结论:EGF通过刺激囊胚期细胞分化而促进胚胎体外发育。但高浓度EGF抑制胚胎体外发育并使早期胚胎受损。
Objective:To evaluate influence of epidermal growth factor on the development of
mouse preimplantation embryo in vitro.Methods:Tow-cell mouse embryos were cultured in the CZB
media supplemented with EGF at different concentrations.The number of blastocyst and the cell num-
ber in each blastocyst were examined 138 hours after human chorionic gonadotropin injection.Results:
Supplementation of CZB media with EGF at the concentration of 0.1ug/ml caused a significant increase
in the rate of expanded blastocyst and hatched blastocyst,however,at the concentration of 100.0ng/ml,
the percentage of expanded blastcyst and hatched blastcyst was lower than that of other groups and
some cells in the blastcyst had regressed.Conclusion:EGF can promote the developing of mouse
preimplantation embryo in vitro by stimulating the cell differentiation.
出处
《山东大学学报(医学版)》
CAS
2004年第2期150-152,158,共4页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金(Y99C01)
关键词
表皮生长因子
小鼠
胚胎
体外发育
Epidermal growth factor
Mice
Embryo
Developing in vitro