转入EGFR cDNA对bcl-2及Bax在胶质母细胞瘤细胞内表达的影响

Influence of transfected EGFR-cDNA on bcl-2 and Bax in glioblastoma cells

目的 探讨bcl 2及Bax蛋白与胶质母细胞瘤细胞系U87核基质的关系 ,以及导入EGFRcDNA对该蛋白在U87细胞内表达的影响。方法 运用共振聚焦显微镜、Western杂交方法分析bcl 2、Bax蛋白与U87细胞核基质的相关性 ,以及将EGFRcDNA转入U87细胞后bcl 2及Bax表达的改变。结果 bcl 2位于细胞核基质的周边 ,Bax位于核基质内。用Western杂交可在核基质蛋白内检测出一条 2 6 0 0 0的bcl 2带及一条约 6 6 0 0 0特异性Bax带。在EGFRcDNA转入的U87细胞中 ,Bax表达较对照组增强 ,而bcl 2的表达则较对照组减弱。结论 bcl 2及Bax蛋白是核基质相关蛋白。bcl 2及Bax基因可能参与EGFRcDNA所诱导的胶质母细胞瘤细胞的转化过程。

Objective To investigate the correlation of bcl 2 and Bax protein with nuclear matrix in glioblastoma cell line U87 as well as the effect of EGFR cDNA transfection on the expression of bcl 2 and Bax in U87 cells. Methods The correlation of bcl 2 and Bax protein with nuclear matrix in glioblastoma cell line U87 was studied by confocal microscopy and Western blot. The expression of bcl 2 and Bax in EGFR cDNA transfected and untransfected glioblastoma cell lines was studied by Western blot. Results Confocal microscopic images showed that bcl 2 protein was localized in the periphery of the nuclear matrix and Bax in the nuclear matrix. A 26 kDa bcl 2 band and a specific band of Bax at about 66 000 were detected in nuclear matrix proteins by western blot. The expression of bcl 2 was lower but that of Bax was higher in EGFR cDNA transfected cells than the control. Conclusion Bcl 2 and Bax, being nuclear matrix associated proteins, are probably involved in the EGFR cDNA induced malignant conversion of glioblastoma cells by introducing EGFR cDNA into the tumor cells. [