E-cadherin基因转染对膀胱癌细胞生长及浸润能力的影响

Effects of E-cadherin gene transfection on bladder cancer cell growth and invasion

目的 利用转基因技术 ,观察E cadherin(ECD)对膀胱癌生长和浸润转移能力的影响。方法 将细胞间粘附分子ECD真核表达载体转染具有高浸润转移能力的膀胱癌细胞系Ts3L ,测定细胞的生长曲线、克隆形成能力、体外浸润能力及细胞的周期分布。结果 ECD基因转染后的膀胱癌细胞生长速度明显减慢 ,达 3 5 7%;凋亡细胞比例明显增加 ,超高倍体细胞比例明显减少 ;Ts3L/ECD细胞克隆形成能力显著降低 (P <0 0 1) ;Ts3L/ECD细胞的浸润能力明显低于其母代及对照细胞。结论 ECD对肿瘤的生长和浸润转移都有显著的抑制作用 。

Objective To observe the effects of E cadherin (ECD) on bladder cancer cell growth and invasion using gene transfection technique. Methods Human E cadherin gene was transfected into a highly invasive and metastatic bladder cancer cell line Ts3L. Cell growth curves, clone formation rate, in vitro tumor cell infiltrating ability, and cell cycle distribution were determined, respectively. Results Speed of bladder cancer cell growth decreased significantly to 35 7% after ECD gene transfection. Apoptotic cell rate in ECD transfected cells was markedly higher than that of the control cells. The percentage of superploid cells in Ts3L/ECD cells was significantly reduced than that in the control cells. Clone formation rate of ECD transfected cells, detected by soft agarose gel clone formation test, was significantly decreased as compared with that of the control cells ( P <0 01). In vitro tumor cell infiltrating ability was markedly suppressed in Ts3L/ECD cells, as compared with that of its parental and control cells ( P <0 01). Conclusion ECD might play an important role in the inhibition of bladder cancer cell growth and invasion. It is an important inhibitory factor for tumor growth and invasion of bladder cancer.