血管内皮生长因子在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of vascular endothelial growth factor in human non-small cell lung cancer

目的 研究血管内皮生长因子的表达与非小细胞肺癌临床病理特征及预后的关系 ,探讨血管内皮生长因子的作用机制。方法 采用超敏过氧化酶免疫组织化学法 ,检测手术切除的 96例非小细胞肺癌组织标本中微血管密度和血管内皮生长因子的表达。结果 96例非小细胞肺癌组织标本中血管内皮生长因子的阳性表达率为 64 .6% ( 62 /96) ,主要分布于癌细胞的胞浆内 ,细胞核内无表达。VEGF表达与临床分期有密切关系 (P =0 .0 41) ,与其他各项临床病理特征无明显统计学相关性 (P >0 .0 5 )。微血管高密度组血管内皮生长因子阳性表达率为 80 .4% ,明显高于微血管低密度组 ( 4 6.7% ) (P =0 .0 0 1)。血管内皮生长因子阳性表达患者生存期明显短于阴性表达患者 (P <0 .0 1)。Cox风险比例模型分析显示血管内皮生长因子表达和病理分期可作为判断非小细胞肺癌患者预后的独立指标。结论 血管内皮生长因子可引起瘤内微血管密度增加 ,在非小细胞肺癌血管形成中起重要作用 ,并有助于预测肺癌患者预后。

Objective To investigate the expression of vascular endothelial growth factor (VEGF) and its relation to clinical characteristics and prognosis of non-small cell lung cancer (NSCLC), and to study the possible mechanism of VEGF. Methods The expression of VEGF and intratumoral microvascular density (MVD) were determined in 96 NSCLC tissues by SP immunohistochemical method. Results VEGF was mainly stained in the cytoplasm of tumor cells. The positive rate of VEGF expression was 64.6% in 96 patients. The positive rate of VEGF expression was related to stages (P=0.041), but not to other clinical characteristics of NSCLC (P>0.05). The expression of VEGF in high MVD group (80.4%) was significantly higher than that in low MVD group (46.7%) (P=0.001). The patients with positive VEGF expression had a significantly shorter survival duration than those with negative VEGF expression (P<0.01). By Cox proportional hazard model analysis, the expression of VEGF and clinical stage were considered the independent predictors for the prognosis of NSCLC. Conclusion The results suggest that VEGF plays an important role in the angiogenesis of NSCLC, and that detection of VEGF expression may be helpful to predict prognosis of NSCLC.