摘要
目的 :探讨超声引导下大鼠肝癌模型的制作方法。方法 :用60 Co照射和氢化可的松联合免疫抑制 13只Wis tar大鼠。将CBRH 7919大鼠肝癌细胞悬液注入 1只大鼠皮下 ,2周后将生成的肿瘤组织剪成小瘤块 ,匀浆后制成细胞悬液 ,在超声引导下将细胞悬液接种于 12只大鼠肝脏 ,并用超声观察肿瘤生长。 2周后处死动物 ,进行病理学检查。结果 :12只肝脏种植的大鼠中有 1只在实验中死亡 ,接种 2周后超声检查发现 10只肝内有肿块生成 ,1只肝内接种失败。病理类型均为肝细胞性肝癌。大鼠肝癌原位模型成功率为 83 .3 %。结论 :超声引导下在免疫抑制大鼠内穿刺接种制作大鼠肝细胞癌模型 ,是一种操作方便、简单、成功率高的方法 ,便于肝癌影像学的诊断和治疗。
Objective:To develop a method to establish the liver cancer model in immune suppressed rats under ultrasound guidance.Methods:The immune system of a rat was suppressed by 60 Co irradiation and intraperitoneal injection of Hydrocortisone,then the rat received a subcutaneous inoculation of CBRH 7919 rat liver cancer cells. After 2 weeks the grown tumor was cut into pieces in strip shape, and they were inoculated into the liver of 12 immune suppressed rats under ultrasound guidance.The tumor growth in the model was observed using ultrasonography. About 2 weeks after transplantation,the tumors were taken for pathological examination.Results:1 out of 12 rats was dead during transplantation.About 2 weeks after inoculation, ultrasonography showed that liver tumors grew in 10 rats and failed to grow in only one. The pathological examination showed that the tumors were hepatocellular carcinoma. The success rate of tumor inoculation was 83.3%.Conclusion:The ultrasound guiding inoculation method for establishing liver cancer in immune suppressed rats is easy, simple and effective.
出处
《放射学实践》
2004年第3期174-175,共2页
Radiologic Practice
