摘要
目的 建立测定翠莲解毒片中脱水穿心莲内酯含量的 HPL C方法。方法 色谱柱为 Diamonsil- C1 8柱 ,甲醇 -水 (80∶ 2 0 )为流动相 ,流速 0 .8m L / min,检测波长 2 2 5 nm ,柱温为室温。结果 脱水穿心莲内酯在18.9~ 132 .3μg/ m L范围内线性关系良好 ,相关系数 r=0 .9999。脱水穿心莲内酯的平均回收率为 98.3% ,RSD为 2 .78%。结论 所用方法简便、准确 。
Objective A RP-HPLC method for the determination of 14-deoxy-11,12-didehydroandrographolide in Cuilianjiedu Tablets was established.Methods A Diamonsil C 18 column was used,with the mobile phase of methanol-water(80∶20).The flow rate was 0.8 mL/min, and the detection wavelength was 225 nm.Results The linear ranges of 14-deoxy-11,12-didehydroandrographolide was 18.9~132.3 μg/mL(r=0.999 9). The average recovery of 14-deoxy-11,12-didehydroandrographolide was 98.3% with RSD 2.78%.Conclusion The method is simple, accurate and reliable for quality control of this preparation.
出处
《现代中药研究与实践》
CAS
2004年第1期43-45,共3页
Research and Practice on Chinese Medicines