乙型肝炎患者外周血T淋巴细胞活化的研究

Study on activated peripheral T lymphocyte in HBV infected patients

目的 :探讨HBV感染不同的临床结果以及HBV -DNA的复制与其外周血中淋巴细胞活化状况的关系。方法 :应用流式细胞技术检测187例乙型肝炎患者及16例健康对照外周血中淋巴细胞的活化 ,同时利用实时荧光定量PCR测定其体内HBV -DNA载量。结果 :以HLA -DR作为各T淋巴细胞的活化指标 ,急性肝炎恢复期和慢性肝炎患者活化的CD3+、CD4+和CD8+淋巴细胞明显高于健康对照和慢性病携带者 (P<0.05或P<0.01)。而在慢性肝炎中。病程越严重 ,其外周血淋巴细胞的活化率亦越高。但乙型肝炎患者体内的病毒载量与其外周血淋巴细胞的活化无明显相关性 (P>0.5)。结论 :乙型肝炎患者所致的肝细胞损坏以及其体内病毒的清除与机体的免疫状况明显相关 ,但体内乙型肝炎病毒的复制与机体的免疫功能有关。

Objective:To evaluate the relationship between the peripheral Tlymphocyte activation and different clinical results or HBV replication.Methods:Peripheral Tlymphocyte actication was tested by flowcytomety(FCM)in187cases of HBV infected patients and16healthy subjects,while the HBV-DNA load was detected by real time fluorescence PCR in the same samples.Results:We analysised HLA-DR positive as activated lymphocytes.HLA-DR expressions on CD3 + ?CD4 + and CD8 + Tlymphocyte from acute and chronic hepatitis were more high than that fromhealthy control and HBV carrier(P<0.05or P<0.01).Among the chronic hepatitis,the more severe of the clinic,the higher rate of the lymphocyte activation.On the other hand,there was no relationship between viral load and the lymphocyte activation(P>0.05).Conclusion:The hepatocyte damage and the virus clean is dependant on host immune function,while there is no relationship between the viral replication and host immune function.