大鼠单核/巨噬细胞功能异质性及其在创伤失血后免疫紊乱中的意义

Functional heterogeneity of monocytes/macrophages and its significance in immune dys function after trauma-hemorrhage in rats

目的 探讨单核 /巨噬细胞功能异质性与创伤后免疫紊乱的关系。方法 健康Wistar大鼠 48只 ,分为正常对照组、创伤 失血后 1,4,7d组 ,以无色孔雀绿比色法及流式细胞仪分别测定不同部位单核 /巨噬细胞非特异性吞噬功能及I A抗原的表达。结果 ①生理状态下 ,肺泡巨噬细胞 (AM)非特异性吞噬功能最强 ,腹腔巨噬细胞 (PM )次之 ,单核细胞 (Mo)最弱。PM高表达I A ,而AM及Mo表达量较低。②创伤 失血后大鼠单核 /巨噬细胞非特异性吞噬功能均下降 ,但各部位变化具有差异性。AM降低最为显著 ,且持续时间长 ,而PM受影响较小。创伤 失血后大鼠单核 /巨噬细胞I A抗原表达变化各异 ,PM伤后持续处于较低水平 ,而AM在伤后 4、7d出现升高。结论 生理状态下单核 /巨噬细胞功能及表型存在异质性 ,创伤 失血后这种异质性表现更为明显 。

Objective To investigate the relationship between the functional heterogeneity of monocytes/macrophages and the immune dysfunction after severe trauma. Methods A total of 48 healthy Wistar rats were divided into control group and post trauma hemorrhage group. The non specific phagocytotic abilities and the expression of I A of differently distributed monocytes/macrophages were evaluated by benzaldehyde green assay and flow cytometry, respectively. Results (1) In the control group, alveolar macrophages (AM), peritoneal macrophages (PM), monocytes (Mo) showed a great functional difference in phagocytosis and expression of I A; (2) After trauma hemorrhage, the nonspecific phagocytotic ability of AM, PM, and Mo isolated from the injured rats declined significantly, but that of AM decreased most significantly and lasted longer. The changes of I A antigen expression of monocytes/macrophages after trauma hemorrhage varied. The nonspecific phagocytotic ability of PM remained at a very low level after trauma, but that of AM began to increase at 4 and 7 d after trauma. Conclusion There is phenotypic and functional heterogeneity of monocytes/macrophages under normal condition. The heterogeneity is more obvious after trauma hemorrhage, which may play an important role in the immune dysfunction and the development of complications after trauma.