摘要
A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluorescence signal quickly and quantitatively. This method provides effective and original approach to researching the dynamic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.3×10?4 U/mL to 0.23 U/mL. It is rapid and sensitive to detect 2.8×10?5 U T4 DNA ligase in 10 min.
基金
国家重点基础研究发展计划(973计划)，科技部"攀登计划"，国家自然科学基金，国家杰出青年科学基金，教育部重点项目，教育部高校骨干教师资助计划，国家海外青年学者联合基金，湖南省自然科学基金
