梭曼中毒诱导PC_(12)细胞JAKs表达

JAKs expressions in cell line PC_(12) induced by soman intoxication

目的 研究梭曼中毒PC1 2 细胞中JAK1、JAK2、JAK3基因mRNA和蛋白表达的变化。方法 采用半定量RT PCR和Westernblotting法检测梭曼中毒 2、6、12、2 4h的PC1 2 细胞的JAK1、JAK2、JAK3基因mRNA和蛋白质的表达水平 ,并对PCR产物进行测序。结果 梭曼中毒后 2h组PC1 2 细胞中JAK1、JAK2、JAK3mRNA和蛋白质表达水平升高 ,在中毒后 12h组达最高 ,中毒后 2 4h组的表达量低于 12h组 ,与对照组比较均有明显差别。RT PCR产物直接测序证实扩增产物片断与GenBank中相应序列相同。结论 梭曼中毒增强PC1 2 细胞中JAKs基因的表达 。

Objective To investigate the effect of soman on the Janus kinases (JAKs) expression in cell line PC 12 . Methods The PC 12 cell was used in these experiments and treated with soman at a concentration of 20 μmol/L . RT PCR and Western blotting were employed to detect the mRNA and protein expressions of JAK1, JAK2 and JAK3 at the time points of 0, 6, 12 and 24 h. The products were sequenced by Sanger's double strand DNA sequence determination. Results The expression levels of JAK1, JAK2 and JAK3 mRNAs and proteins increased at 2 h, reached the highest at 12 h and decreased at 24 h, but they were still higher than those of the control. It was shown that the sequences of amplification products by RT PCR were the same to corresponding ones in GenBank. Conclusion Soman intoxication enhances the expression of Janus kinases in PC 12 cells. JAKs genes may play an important role in brain injury due to soman intoxication.