p63基因在小细胞肺癌及肺腺癌组织中转录表达水平的研究

Expression of p63 gene in small cell lung carcinoma and lung adenocarcinoma

目的 研究p63基因在人类小细胞肺癌 (Small celllungcarcinoma ,SCLC)及肺腺癌组织中转录表达水平及其意义。方法 采用RT PCR(Reversetranscriptase PCR)方法对 6例小细胞肺癌及 15例肺腺癌组织中p63基因两种亚型(TAp63及ΔNp63 )转录表达情况进行检测和比较 ,同时用免疫组化检测p63蛋白在上述组织中的表达情况。结果 5例SCLC肿瘤组织及 1例SCLC癌旁组织中ΔNp63呈阳性表达 ,所有SCLC肿瘤、癌旁及远癌肺组织均无明显TAp63表达 ;腺癌肿瘤、癌旁及远癌肺组织TAp63与ΔNp63表达均为阴性 (RT PCR)。p63蛋白在SCLC组织中的阳性表达率和表达强度明显大于腺癌组织 (免疫组化 ) (P <0 .0 1)。结论 p63基因在小细胞肺癌中呈阳性表达 ,其中主要是ΔNp63表达水平升高 ,而p63蛋白在SCLC中表达水平明显高于腺癌组织 ,这可能与其在小细胞肺癌发生、发展中所起的调控作用有关 ;推测ΔNp63基因在小细胞肺癌组织中转录表达水平升高可能抑制细胞凋亡 ,促进肿瘤增殖 。

Objective To investigate the significance of p63 gene expression in small cell lung carcinoma(SCLC) and lung adenocarcinoma. Methods Reverse transcriptase polymerase chain reaction (RT PCR) technique was used to observe p63 gene expression(TAp63 and ΔNp63) in 6 cases of SCLC and 15 cases of lung adenocarcinoma. The p63 protein expression was also observed imunohistochemically. Results Over expressed ΔNp63 was found in 5 cases of SCLC and in tumor adjacent tissues in 1 case of SCLC. There was no expression of TAp63 in SCLC, tumor adjacent and tumor free tissues. RT PCR showed negative expressions of TAp63 and ΔNp63 in adenocarcinoma, tumor adjacent and tumor free tissues. Imunohistochemical staining revealed that the positive staining rates and intensity of p63 protein in SCLC were significantly higher than those in lung adenocarcinoma ( P <0.01). Conclusion There is positive expression of p63 gene in SCLC, which may be related to the function of p63 in genesis and development of SCLC. The overexpression of ΔNp63 in SCLC can suppress the apoptosis and accelerate the proliferation of tumor, so ΔNp63 may be an oncogene amplified in SCLCs.