影响MCF-7细胞增殖试验相关因素的初步研究

Study of the related influencing factors of MCF-7 cells proliferation test in vitro

目的 了解影响MCF 7细胞增殖试验相关因素 ,为MCF 7细胞增殖试验检测环境雌激素方法的标准化提供试验依据。方法 对不同来源的MCF 7细胞雌激素敏感性进行检测 ,对筛选出的敏感细胞在不同培养条件、去激素培养基中培养不同时间进行细胞增殖试验。结果 不同来源MCF 7细胞对雌激素敏感不同 ;培养基中酚红、血清中内源性雌激素对MCF 7细胞有刺激增殖作用 ;MCF 7细胞在去激素培养基中培养时间越长 ,对雌激素越敏感。结论 进行MCF 7细胞增殖试验前应进行雌激素敏感试验 ,选用敏感细胞 ;

Objective To study the related influencing factors of MCF 7 cells proliferation test in vitro to provide some criteria for the standard detection of environmental estrogens (EE) by cell proliferation test of MCF 7 cells in vitro . Methods The sensitivity to E 2 stimulation of different sources of MCF 7 cell lines was detected. Cell proliferation test was performed on the screened sensitive cells in different culture conditions. Results In the estrogen free medium, C cell line of MCF 7 was more sensitive to E 2 stimulation than A and B cell lines ( P <0.01). The phenolsulfonphthalein in medium and the endogenous estrogen in NBS had stimulating effect on the cell proliferation of MCF 7. The longer MCF 7 was cultured in estrogen free medium, the more sensitive was the cell to the stimulation of estrogen( P <0.01). Conclusion Evaluation of the estrogenic activity by MCF 7 cell proliferation test in vitro may be influenced by many factors.