环氧合酶-2及其特异性抑制剂NS-398对膀胱癌细胞体外生长与侵袭能力的影响

Effects of cyclooxygenase-2 and its specific inhibitor NS-398 on the growth and invasion ability of urothelial carcinoma cell line EJ

目的 研究环氧合酶 2 (Cyclooxygenase 2 ,COX 2 )及其特异性抑制剂NS 3 98在膀胱癌细胞生长和侵袭转移中的作用。方法 利用转基因技术将COX 2cDNA基因导入低表达COX 2的膀胱癌细胞株EJ ,获得高表达COX 2的永久转染细胞EJ COX2 。观察转染前后细胞生长速度的变化 ;在NS 3 98作用下 ,Boyden小室检测细胞的侵袭能力的改变 ,用RT PCR和Westernblotting检测uPA的表达变化。结果 转染COX 2的EJ COX2 细胞体外增殖能力增强 ;穿过人工基底膜的体外侵袭能力增强 ;尿激酶型纤溶酶原激活因子 (Urokinaseplasminogenactivator ,uPA)的表达上升 ;NS 3 98能呈剂量依赖地抑制COX 2、uPA的表达和EJ COX2 细胞的侵袭能力。结论 COX 2能促进膀胱癌细胞生长 ,并通过促进uPA的表达从而促进其侵袭转移的能力 ,NS 3 98能呈剂量依赖地抑制COX 2、uPA的表达和EJ COX2 细胞的侵袭能力。

Objective To study the function of cyclooxygenase 2(COX 2) and its specific inhibitor NS 398 on the cell growth and invasion ability of urothelial carcinoma cell line EJ. Methods The cox 2 cDNA was transfected into the urothelial carcinoma cell line EJ and a cell line EJ COX 2 which highly expressed cox 2 gene permanently was gained. The cell growth rate before and after transfection was observed. Then at various concentrations of NS 398, the invasion ability was detected by Boyden Chamber and expression levels of uPA by RT PCR and Western blot. Results The EJ COX 2 cell line grew more rapidly and had a stronger invasion ability than EJ and its uPA expression increased significantly. NS 398 could dose dependently inhibit the expressions of COX 2 and uPA and the invasiveness of EJ COX 2 cell. Conclusion COX 2 can stimulate the growth of urothelial cell line EJ and promote its invasion ability by stimulating the expression of uPA.