慢性捆绑应激对大鼠学习记忆及海马I-B4表达的影响

Effect of the Chronic Restraint Stress upon Learning and Memory and upon Expression of I-B4 in Hippocampus

目的探讨小胶质细胞在应激中的作用。方法把大鼠分为应激组和正常对照组 ,应激组大鼠每天捆绑6h ,持续 2 1d ,然后用Y迷宫测试两组大鼠学习记忆能力 ,应用免疫组织化学方法测定两组大鼠海马小胶质细胞I-B4的表达。结果应激组大鼠学习记忆能力明显受损 (P <0 .0 5 ) ,其海马小胶质细胞I -B4免疫阳性产物较对照组明显增加 (P <0 .0 5 )。结论慢性捆绑致大鼠学习记忆受损可能与海马小胶质细胞I-B4表达增加有关。

Objective To explore the role of microglia in chronic restraint stress. Methods Rats were divided into stress and control groups. The stress rats were restrained for 6 hours every day for 21 days, and then the behavior of rats in two groups was tested respectively by Y-maze and the expression of the I-B4 in hippocampus of those rats in two groups were observed by immunocytochemical method. Results The learning and memory ability of the stress rats was impaired (p<0.05). The immunoreactivities of the I-B4 were increased in hippocmpal of the stress rats (p<0.05).Conclusion The impairment of learning and memory ability of the stress rats might be related to the change of the I-B4 in hippocampal microglia of the rats.