流式细胞术在富营养淡水湖泊微型浮游植物细胞中的应用

Studies on Nanophytoplankton Cell Using Flow Cytometry (FCM) in a Eutrophic Lake

应用流式细胞术(FCM)对一个富营养化淡水湖泊表、底层微型浮游植物细胞进行了初步研究。研究结果表明:流式细胞术可快速、多参数区分3种不同类群微型浮游植物。微型浮游植物细胞在表、底层占50μm以下微型颗粒物数量比例分别为21.08％、17.87％,在不同水层,微型浮游植物的优势类群及数量也不同。流式细胞术大大提高了淡水微型浮游生物研究监测水平。

Nanophytoplankton cell was preliminary studied by using flow cytometry (FCM) at surface and bottom layers in a eutrophic lake. Three populations could be rapidly discriminated and enumerated. Nanophytoplankton cell occupied the small part of total particles that were smaller than 50 μm. They represented 21.08% and 17.87% of total particles at surface and bottom layers respectively. FCM considerably improved the method of monitoring and studying on nanophytoplankton.