一种改良的肌细胞骨架染色方法

A Modified Method for Cytoskeleton Staining of Muscle Cells

为了观察肌细胞骨架,对传统考马斯亮蓝染色法进行改良,并与免疫荧光染色法进行了比较。培养的血管平滑肌细胞先用多聚甲醛预固定后再进行考马斯亮蓝染色,可使细胞骨架非常清晰的显色,解决了传统考马斯亮蓝染色易使肌细胞变形、脱片的问题,其效果与免疫荧光染色相近。因此,多聚甲醛预固定-考马斯亮蓝染色法是一种适于肌细胞骨架染色的简便方法。

In this study, we improved classical staining method for cytoskeleton with coomassie brilliant blue R250 and compared it with immunofluorencence. The cultured vascular smooth muscle cells were transiently prefixed with paraformaldehyde, then stained with coomassie brilliant blue as routine procedures. The cytoskeleton stained by the modified method was clear and kept its nature shape. The paraformaldehyde prefixed-coomassie brilliant blue staining is a convenient cytoskeleton staining method for muscle cells.