摘要
目的 探讨核酶P对HCMVUL97mRNA的体外切割能力。方法 以人巨细胞病毒 (humancytomegalovirus ,HCMV)磷酸转移酶mRNA序列为靶设计externalguidesequences(EGS) ,共价结合到大肠杆菌来源M 1RNA中 ,构建成M 1GS-T5核酶。对其进行UL97基因亚克隆片段转录产物的体外切割实验。结果 该核酶在一定离子浓度下具对UL 97mRNA片段产生特异切割能力。结论 新构建得到的核酶MIGS -T5具特异性切割活性 ,可发展为一种抗病毒试剂。
Objective:To explore the cleaved ability of RNase P target HCMV UL97 mRNA in vitro.Methods:RNase P(M1GS-T5) ribozyme was constructed by linking the M1 RNA from Escherichia coli covalently to a guide sequence(EGS) that is complementary to the mRNA coding for UL97 phosphotransferase gene of human cytomegalovirus(HCMV),and was used to cleave the UL97 mRNA segment.Result:the M1GS can efficiently cleaved the UL97 mRNA segments under the certain ion concentration in vitro.Conclusion:the M1GS-T5 that constructed by us has the specific cleaved ability,and can be developed as an antiviral agent.
出处
《中国优生与遗传杂志》
2004年第2期8-10,共3页
Chinese Journal of Birth Health & Heredity
基金
国家自然科学基金 ( 3 0 3 70 776)
广东省自然科学基金重大项目 ( 3 670 3 )
广东省自然科学基金 ( 0 2 1162
0 0 0 718)
关键词
核酶P
引导序列
人巨细胞病毒
磷酸转移酶
RNase P
external guide sequence (EGS)
human cytomegalovirus (HCMV)
phosphotransferase
