塞来昔布对人肝癌SMMC-7721细胞的生长抑制作用

Growth-inhibition effect of celecoxib on human hepatocellular carcinoma cell line SMMC-7721

目的 研究环氧合酶 2 (cyclooxygenase 2 ,COX 2 )抑制剂塞来昔布对人肝癌SMMC 772 1细胞的生长抑制作用。方法 用MTT比色法观察其对肝癌细胞生长的影响 ;荧光显微镜和透射电子显微镜检测细胞凋亡 ,TUNEL染色法记数细胞凋亡指数 ,流式细胞仪定量分析。结果 塞来昔布以剂量依赖的方式抑制SMMC 772 1细胞的生长 ,2、10、2 0及 40mmol L塞来昔布对肝癌细胞的生长抑制率分别为 2 0 78%、3 3 3 7%、48 5 7%和 64 96%(P <0 0 1)。荧光显微镜和透射电镜观察到细胞皱缩、核质浓缩、核碎裂以及凋亡小体形成等凋亡形态学改变。流式细胞仪定量分析示 2、10、2 0及 40mmol L浓度下细胞凋亡率分别为 ( 7 44± 0 3 4) %、( 19 5 9± 1 73 ) %、( 2 9 0 4± 4 18) %和 ( 4 2 14± 2 40 ) %,与对照组 ( 2 13±0 17) %比较均有显著性差异。结论 塞来昔布能抑制人肝癌SMMC 772 1细胞增殖 ,亦能诱导其凋亡 ;

Objective To study the inhibitory effect of cyclooxygenase 2 (COX 2) inhibitor celecoxib on the growth of hepatocellular carcinoma cell (HCC) line SMMC 7721 in vitro . Methods Growth suppression was evaluated by MTT method. Apoptosis related changes in morphology were observed by fluorescence microscopy (FM) and transmission electron microscopy (TEM). Apoptotic index (AI) was counted by the TDT mediated dUTP biotin nick end labeling (TUNEL) assay. The apoptotic rate was quantified by flow cytometry (FCM). Results Celecoxib inhibited the growth of HCC cells in a dose dependent manner. The growth inhibitory rates of HCC cells treated with 2, 10, 20, and 40 mmol/L celecoxib were 20.78%, 33.37%, 48 57%, and 64 96%, respectively ( P <0.01). Apoptosis with nuclear chromatin condensation, fragmentation, cell shrinkage, and the formation of apoptotic bodies was found by fluorescent staining and TEM. Apoptotic rates of HCC cells treated with 2, 10, 20, and 40 mmol/L celecoxib were (7.44±0.34)%, (19.59±1.73)%, (29.04±4.18)%, and (42.14±2.40)%, respectively, significantly higher than those of the control group (2.13±0.17)% ( P <0.05). Conclusion Celecoxib can inhibit the growth of human hepatocellular carcinoma cell line SMMC 7721 as well as induce the cell apoptosis which may be related to the mechanism of inhibition of HCC cells.