化疗药物对胰腺癌细胞株Aspc-1和Bxpc-3生长抑制作用的研究

Study on growth inhibitory effect of chemotherapeutic drugs on the pancreatic cancer cell line-Aspc-1 and Bxpc-3

目的 探讨不同化疗药物浓度、作用时间及联合用药对胰腺癌细胞株 (Aspc 1和Bxpc 3)的生长抑制作用。方法 单独或联合使用不同浓度的 4种化疗药物 :5 氟尿嘧啶、丝裂霉素、顺铂和表柔比星 (浓度分为d1、d2、d3、d4 ) ,分别作用 2 4、4 8和 72h后 ,用四唑盐比色法检测药物对两株细胞的生长抑制作用 ,并对计算出的每组细胞生长抑制率行t检验分析。结果 两细胞株的生长抑制率随着药物浓度的增加和作用时间的延长而显著上升 (P <0 .0 5 ) ,联合用药能显著提高药物对两细胞株的抑制作用 (P <0 .0 5 )。结论 通过动脉灌注或保留导管持续灌注以提高化疗药物局部浓度和延长药物作用时间 。

Objective To study the growth inhibitory effect of chemotherapeutic drugs on the pancreatic cancer cell line Aspc-1 and Bxpc-3, and the relations to the varied concentration and exposere time of chemotherapeutic drugs administrated in single or in combination. Methods Four chemotherapeutic drugs included 5-fluorouracil (5-FU), epirubicin (E-ADM), mitomycin(MMC) or cisplatin (DDP) administrating in single or in combination and using varied concentrations(d1、d2、d3 and d4) and exposure times(24、28 and 72 hours); and then the growth inhibitory effect on the two cell lines. resulted from these drugs were assayed by MTT colorimetry and analysed by “ t ” test. Results The growth inhibitory rate of two cell lines was increased, with the increment of drug concentration and/or the prolongation of exposure time ( P< 0.05).The chemotherapeutic drugs administrated in combination could obviously increase the growth inhibitory effect on the two cell lines. than those with single application( P <0.05). Conclusion The results indicated that increment of the local drug concentration and prolongation of the exposure time by intraarterial infusion or consecutive arterial infusion through the retained catheter, as well as the combined administration of the chemotheraputic drugs, can improve the chemotherapautic efficiency for pancreatic carcinoma.