圆二色光谱揭示光系统Ⅱ在热处理过程中叶绿素荧光动力学变化的原因
摘要
研究了光系统Ⅱ(PSⅡ)在热处理过程中的叶绿素α荧光和圆二色(CD)光谱。在30℃~40℃热处理过程中,PSⅡ的叶绿素荧光参数Fo′保持稳定不变;当温度大于40℃时,Fo′逐渐升高并在55℃达到最大值。在PSⅡ颗粒和富含捕光色素(LHCⅡ)的复合物的热处理过程中,具有超大振幅的CD异常信号出现,并且在40℃时,677nm的异常CD峰强度达到最大。这些结果暗示在PSⅡ颗粒热处理过程中,PSⅡ颗粒中的LHCⅡ的聚集状态和异常CD信号相关,并且也可能是影响Fo′的一个重要因素。
参考文献20
-
1[1]Barzda V, Mustardy L, Garab G. 1994. Size dependency of circular dichroism in macroaggregates of photosynthetic pigmentprotein complexes. Biochemistry, 33:10837-10841.
-
2[2]Berry J, Bjorkman O. 1980. Photosynthetic response and adaptation to temperature in higher plants. Ann Rev Plant Physiol,31:491-453.
-
3[3]Bukhov N G, Sabat S C, Mohanty P. 1990. Analysis of chlorophyll a fluorescence changes in weak light heat-treatedAmaranthus chloroplast. Photosynth Res, 23:81-87.
-
4[4]Garab G, Kieleczawa J, Sutherland J C, Bustamante C, Hind G.1991. Organization of pigment-protein complexes into macrodomains in the thylakoid membranes of wild type and chlorophyll b-less mutant of barley as revealed by circular dichroism.Photochem Photobiol, 45:273-281.
-
5[5]Garab G, Wells K S, Finzi L, Bustamante C. 1988. Helicallyorganized macroaggregates of pigment-protein complexes inchloroplasts: evidence from circular intensity differentialscattering. Biochemistry, 27:5839-5843.
-
6[6]Gregory R P F, Demeter S, Faludi-Daniel A. 1980. Macromolecular organization of chlorophyll a in aggregated chlorophyll a/b protein complexes shown by circular dichroism at room andcryogenic temperatures. Biochim Biophys Acta, 591:356-360.
-
7[7]Keller D, Bustamante C. 1986. Theory of the interaction of lightwith large inhomogeneous aggregates. Ⅱ. Psi-type circulardichroism, J Chem Phys, 84:2972-2980.
-
8[8]Kuwabara T. Murata N. 1982. Inactivation of photosyntheticoxygen evolution and concomitant release of three polypeptides in the photosystem Ⅱ particles of spinach chloroplasts.Plant Cell Physiol, 23:533-539.
-
9[9]Livolant F, Maestre M F. 1988. Circular dichroism microscopyof compact forms of DNA and chromatin in vivo and in vitro:cholesteric liquid-crystalline phases of DNA and single dinoflagellate nuclei. Biochemistry, 27:3056-3068.
-
10[10]Lou SQ(娄世庆),WangKB(王可玢),ZhaoFH(赵福洪),XuC H(许春辉),Kuang T Y(匡廷云).1995.A comparativestudy on PS Ⅱ light harvesting chlorophyll a/b protein complexes between spinach and cucumber.Acta Bot Sin(植物学报),37:192-197.
-
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-
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