自发性高血压大鼠下丘脑视上核一氧化氮合酶与加压素的共表达

Co-Existence of Nitric Oxide Synthase Positive Neurons and Vasopressin in the Supraoptic Nucleus of the Spontaneously Hypertensive Rat

目的:观察自发性高血压大鼠(SHR)与正常大鼠下丘脑视上核(SON)内一氧化氮合酶(NOS)与加压素(AVP)在神经元内的分布和共存。方法:运用NADPH-d组织化学方法,结合ABC免疫组织化学技术。结果SON内NOS阳性神经元与AVP免疫阳性神经元的分布与形态基本类似,并高度共存:SHR组NOS-AVP双标记阳性神经元约占阳性标记神经元总数的62.7％,主要分布于SON的腹侧部;正常SD对照组NOS-AVP双标记阳性神经元约占阳性标记神经元总数的72.3％。结论:实验结果提示一氧化氮(NO)与AVP在下丘脑的血压神经内分泌调节活动中起着重要的介导作用,以及对高血压的发生发展也可能有影响。

Objective: The co-existence of nitric oxide synthase(NOS) positive neurons and vasopressin was to be investigated in Supraoptie nucleus (SON) of Spontaneously hypertensive rat(SHR) and normal rat. Methods: The morphology and distribution of NOS positive neurons and AVP immunoreactive neurons were observed respectively in SON of both SHR and normal rat, and the co-existence of NOS with AVP was also observed by using NADPH-diaphorase histochemical technique combined with immunocytochemical methods in the same section. Results: The morphology and distribution of NOS positive neurons and AVP immunoreactive neurons were fundamentally similar in the SON, and the ratio of co-existence of NOS-AVP was very high. The percentage of NOS and AVP double -labeled neurons to total labeled neurons in SON of SHR hypothalamus was about 62. 7%. Doubled-labeled neurons were mainly located in the ventral part of the SON. And the percentage of NOS and AVP double-labeled neurons to total labeled neurons in SON of normal rat hypothalamus was about 72. 3%. Conclusion: These results suggest that NO and AVP may play an important role in modulating blood pressure of the hypothalamic neuroendocrine regulations, and may be related to the occurrence and development of hypertension.